abstract：:The whole encoding sequence for Yersinia pestis LcrV antigen was cloned into pET-32a(+) and expressed in Escherichia coli BL21 (DE3). The LcrV was high level expressed in the E. coli cytoplasm in a completely soluble form. Recombinant LcrV could be purified from the supernatant of the bacteria lysate after chromatogra...

journal_title：Protein expression and purification

authors： li J,Li B,Li G,Ren J,Zhang J,Xu C,Yang X,Liu S,Fu L,Chen W

更新日期：2008-02-01 00:00:00

abstract：:Antibacterial peptides from various sources express different antibacterial activity. In order to obtain a high activity antibacterial peptide, the sequences of four antimicrobial peptides--Protegrin-1, 4 kDa Scorpion Defensin, Metalnikowin-2A and Sheep Myeloid Antibacterial Peptide SMAP-29--were exploited to generate...

journal_title：Protein expression and purification

authors： Niu M,Li X,Wei J,Cao R,Zhou B,Chen P

更新日期：2008-01-01 00:00:00

abstract：:Staphylococcal enterotoxins (SEs) are powerful superantigens that stimulate non-specific T-cell proliferation produced by Staphylococcus aureus and draw considerable attention as ideal drugs for cancer therapy. The filtrate of S. aureus culture has been used as ampul named Staphylococcal enterotoxin C injection in cli...

journal_title：Protein expression and purification

authors： Pan YQ,Ding D,Li DX,Chen SQ

更新日期：2007-12-01 00:00:00

abstract：:Folding and assembly studies with alpha-helical membrane proteins are often hampered by the absence of high-level expression systems as well as by missing suitable in vitro refolding procedures. Experimental constraints and requirements for heterologous expression and in vitro assembly of cytochrome b6 have been exami...

journal_title：Protein expression and purification

authors： Prodöhl A,Dreher C,Hielscher R,Hellwig P,Schneider D

更新日期：2007-12-01 00:00:00

abstract：:Adrenal cytochrome b561 (cyt b561) is the prototypical member of an emerging family of proteins that are distributed widely in vertebrate, invertebrate and plant tissues. The adrenal cytochrome is an integral membrane protein with two b-type hemes and six predicted transmembrane helices. Adrenal cyt b561 is involved i...

journal_title：Protein expression and purification

authors： Liu W,Rogge CE,Kamensky Y,Tsai AL,Kulmacz RJ

更新日期：2007-12-01 00:00:00

abstract：:Telomerase is a specialized reverse transcriptase that catalyzes the addition of telomeric repeats, TTAGGG in all vertebrates, to the ends of chromosomes. The lack of recombinant purified human telomerase reverse transcriptase (hTERT) has hampered biochemical and structural studies. The primary problem in generating a...

journal_title：Protein expression and purification

authors： Wu CK,Gousset K,Hughes SH

更新日期：2007-11-01 00:00:00

abstract：:The YUH1 gene coding for ubiquitin C-terminal hydrolase 1, a deubiquitinating enzyme, was cloned from the Saccharomyces cerevisiae genomic DNA and expressed in Escherichia coli. YUH1 was fused with the 6 histidine tag at the N-terminus (H6YUH1) or C-terminus (YUH1H6) and purified by an immobilized metal affinity chrom...

journal_title：Protein expression and purification

authors： Yu HA,Kim SG,Kim EJ,Lee WJ,Kim DO,Park K,Park YC,Seo JH

更新日期：2007-11-01 00:00:00

abstract：:The major capsid protein L1 of human papillomavirus (HPV) contains the immunodominant neutralization epitopes of the virus and can auto-assembles to form virus-like particles (VLPs). Therefore, HPV L1 capsid proteins have been well investigated as potential vaccine candidates. To express large quantities of human papi...

journal_title：Protein expression and purification

authors： Ma Z,Chen B,Zhang F,Yu M,Liu T,Liu L

更新日期：2007-11-01 00:00:00

abstract：:Post-translational processing of host defense cathelicidin peptide LL-37 in human sweat and skin generates new antimicrobial peptides. To understand structure and mechanism of action of these LL-37 derivatives, this article presents the cloning and expression of SK-29, KR-20, LL-29, and LL-23. We also provide a two-st...

journal_title：Protein expression and purification

authors： Li Y,Li X,Wang G

更新日期：2007-10-01 00:00:00

abstract：:While well established in bacterial hosts, the effect of coding sequence variation on protein expression in mammalian systems is poorly characterized outside of viral proteins or proteins from distant phylogenetic families. The potential impact is substantial given the extensive use of mammalian expression systems in ...

journal_title：Protein expression and purification

authors： Carton JM,Sauerwald T,Hawley-Nelson P,Morse B,Peffer N,Beck H,Lu J,Cotty A,Amegadzie B,Sweet R

更新日期：2007-10-01 00:00:00

abstract：:Over-expression of heterologous proteins in Escherichia coli is commonly hindered by the formation of inclusion bodies. Nevertheless, refolding of proteins in vitro has become an essential requirement in the development of structural genomics (proteomics) and as a means of recovering functional proteins from inclusion...

journal_title：Protein expression and purification

authors： Qoronfleh MW,Hesterberg LK,Seefeldt MB

更新日期：2007-10-01 00:00:00

abstract：:Serine hydroxymethyltransferase (SHMT) plays a key role in cell physiology as it participates in the different interconversion pathway of folate coenzymes, provides almost exclusively folate one carbon fragments for the biosynthesis of a variety of end products. For the first time, Mycobacterium leprae glyA gene, enco...

journal_title：Protein expression and purification

authors： Sharma S,Bhakuni V

更新日期：2007-09-01 00:00:00

abstract：:Trichoderma reesei tyrosinase TYR2 has been demonstrated to be able to oxidize various phenolic compounds and also peptide and protein bound tyrosine, and thus is of great interest for different biotechnological applications. In order to understand the reaction mechanism of the enzyme it would be essential to solve it...

journal_title：Protein expression and purification

authors： Westerholm-Parvinen A,Selinheimo E,Boer H,Kalkkinen N,Mattinen M,Saloheimo M

更新日期：2007-09-01 00:00:00

abstract：:Advances in expressed protein ligation (EPL) methods that permit specific introduction of unique modifications into proteins have facilitated protein engineering, structure-function and protein interaction studies. An EPL-generated hybrid exchangeable apolipoprotein has been constructed from recombinant fragments of a...

journal_title：Protein expression and purification

authors： Hauser PS,Ryan RO

更新日期：2007-08-01 00:00:00

abstract：:Bovine follicle-stimulating hormone (bFSH) is a pituitary gonadotropin composed of two non-covalently associated polypeptide subunits, which must be glycosylated, folded, and assembled as a heterodimer to be biologically active. Low-level expression of the recombinant bFSH is the factor that limits its usefulness as a...

journal_title：Protein expression and purification

authors： Huo X,Liu Y,Wang X,Ouyang P,Niu Z,Shi Y,Qiu B

更新日期：2007-08-01 00:00:00

abstract：:Transcription factor HIF-1 is a key regulator in cellular adaptation to hypoxia. HIF prolyl hydroxylases (PHDs) control HIF-1 accumulation by hydroxylation dependent on molecular oxygen. Due to this regulation, PHDs have been pointed out as potential drug targets. We have purified catalytically active human PHD3 after...

journal_title：Protein expression and purification

authors： Fedulova N,Hanrieder J,Bergquist J,Emrén LO

更新日期：2007-07-01 00:00:00

abstract：:To produce a monoclonal antibody specific to a mouse major histocompatibility complex (MHC) class II protein, we synthesized the complementary DNAs for the heavy and light chains of a monoclonal antibody by PCR amplification. These cDNAs were then introduced separately into tobacco plant cells. After performing Northe...

journal_title：Protein expression and purification

authors： Hong SY,Kim TG,Kwon TH,Jang YS,Yang MS

更新日期：2007-07-01 00:00:00

abstract：:Introduction and expression of foreign genes in bacteria often results accumulation of the foreign protein(s) in inclusion bodies (IBs). The subsequent processes of refolding are slow, difficult and often fail to yield significant amounts of folded protein. RHG1 encoded by rhg1 was a soybean (Glycine max L. Merr.) tra...

journal_title：Protein expression and purification

authors： Afzal AJ,Lightfoot DA

更新日期：2007-06-01 00:00:00

abstract：:The signal transduction pathway involving the Vav1 guanine nucleotide exchange factor (GEF) and the Rac1 GTPase plays several key roles in the immune response mediated by the T cell receptor. Vav1 is also a unique member of the GEF family in that it contains a cysteine-rich domain (CRD) that is critical for Rac1 bindi...

journal_title：Protein expression and purification

authors： Brooun A,Foster SA,Chrencik JE,Chien EY,Kolatkar AR,Streiff M,Ramage P,Widmer H,Weckbecker G,Kuhn P

更新日期：2007-05-01 00:00:00

abstract：:The full-length human acetyl-CoA carboxylase 1 (ACC1) was expressed and purified to homogeneity by two separate groups (Y.G. Gu, M. Weitzberg, R.F. Clark, X. Xu, Q. Li, T. Zhang, T.M. Hansen, G. Liu, Z. Xin, X. Wang, T. McNally, H. Camp, B.A. Beutel, H.I. Sham, Synthesis and structure-activity relationships of N-{3-[2...

journal_title：Protein expression and purification

authors： Kim KW,Yamane H,Zondlo J,Busby J,Wang M

更新日期：2007-05-01 00:00:00

abstract：:The expression of human lactoferrin in the mammary gland is an attractive approach to diminish its current production cost. Previous attempts to produce lactorferrin in the milk of transgenic animals resulted in very high cost and uncertain results. In this paper, we have directly infused replication-defective adenovi...

journal_title：Protein expression and purification

authors： Han ZS,Li QW,Zhang ZY,Xiao B,Gao DW,Wu SY,Li J,Zhao HW,Jiang ZL,Hu JH

更新日期：2007-05-01 00:00:00

abstract：:NAD(+)-dependent malic enzyme (NAD-ME) gene from Escherichia coli K12 was inserted into an expression vector pET24b(+) and transformed into E. coli BL21 (DE3). Recombinant NAD-ME was expressed upon IPTG induction, purified with affinity chromatography, and biochemically characterized. The results showed that recombina...

journal_title：Protein expression and purification

authors： Wang J,Tan H,Zhao ZK

更新日期：2007-05-01 00:00:00

abstract：:The immunosuppressive drug FK506 binds its targets FK506-binding protein (FKBP) family and modulates cellular processes. Recent studies demonstrated that FK506 shows anti-malaria effects. Newly identified FK506-binding protein 35 from Plasmodium falciparum (PfFKBP35) is assumed to be the molecular target of FK506 in t...

journal_title：Protein expression and purification

authors： Yoon HR,Kang CB,Chia J,Tang K,Yoon HS

更新日期：2007-05-01 00:00:00

abstract：:The membrane-anchored metalloproteinase ADAM17 (TNF-alpha converting enzyme; TACE; EC 3.4.24.86) continues to be an attractive drug target in inflammatory diseases and cancer. Cocrystallization of its catalytic domain with a lead compound was complicated by the tenacious retention of the prodomain that has been shown ...

journal_title：Protein expression and purification

authors： Hoth LR,Tan DH,Wang IK,Wengender PA,Thompson MA,Kamath AV,Geoghegan KF

更新日期：2007-04-01 00:00:00

abstract：:Arginine has been effectively used in various column chromatographies for improving recovery and resolution, and suppressing aggregation. Here, we have tested the effectiveness of arginine as an eluent in dye-affinity column chromatography using Blue-Sepharose, which binds enzymes requiring adenyl-containing cofactors...

journal_title：Protein expression and purification

authors： Arakawa T,Ejima D,Tsumoto K,Ishibashi M,Tokunaga M

更新日期：2007-04-01 00:00:00

abstract：:Phthalate dioxygenase (PDO), a hexamer with one Rieske-type [2Fe-2S] and one Fe (II)-mononuclear center per monomer, and its reductase (PDR), which contains flavin mononucleotide and a plant-type ferredoxin [2Fe-2S] center, are expressed by Burkholderia cepacia at approximately 30mg of crude PDO and approximately 1mg ...

journal_title：Protein expression and purification

authors： Jaganaman S,Pinto A,Tarasev M,Ballou DP

更新日期：2007-04-01 00:00:00

abstract：:G-protein coupled receptors (GPCRs) are seven transmembrane helical proteins involved in cell signaling and response. They are targets for many existing therapeutic agents, and numerous drug discovery efforts. Production of large quantities of these receptors for drug screening and structural biology remains challengi...

journal_title：Protein expression and purification

authors： Bane SE,Velasquez JE,Robinson AS

更新日期：2007-04-01 00:00:00

abstract：:L1 is a human cell adhesion glycoprotein involved in the development of the central nervous system that comprises six immunoglobulin-like domains (Ig1-Ig6), five fibronectin-type III (FN1-FN5) domains, a single transmembrane region and a cytoplasmic domain. It contains 20 potential N-glycosylation sites and is heavily...

journal_title：Protein expression and purification

authors： Gouveia RM,Morais VA,Peixoto C,Sousa M,Regalla M,Alves PM,Costa J

更新日期：2007-03-01 00:00:00

abstract：:Two DNA polymerase genes have been isolated from Thermococcus strains, Thermococcus zilligii from New Zealand, and the other, Thermococcus 'GT', a fast-growing strain isolated from the Galapagos trench. Both genes were isolated by genomic walking PCR, a technique that does not require expression of the gene product. P...

journal_title：Protein expression and purification

authors： Griffiths K,Nayak S,Park K,Mandelman D,Modrell B,Lee J,Ng B,Gibbs MD,Bergquist PL

更新日期：2007-03-01 00:00:00

abstract：:Process development and optimization studies were performed in order to improve the purification process of (rhIFN-gamma). The objective was to generate material with higher purity and quantity. An in-process control screening was developed to obtain the optimal condition for column chromatographic purification by mea...

journal_title：Protein expression and purification

authors： Mohammadian-Mosaabadi J,Naderi-Manesh H,Maghsoudi N,Nassiri-Khalili MA,Masoumian MR,Malek-Sabet N

更新日期：2007-02-01 00:00:00

abstract：:The recently classified group III trypsins include members like Atlantic cod (Gadus morhua) trypsin Y as well as seven analogues from other cold-adapted fish species. The eight group III trypsins have been characterized from their cDNAs and deduced amino acid sequences but none of the enzymes have been isolated from t...

journal_title：Protein expression and purification

authors： Pálsdóttir HM,Gudmundsdóttir A

更新日期：2007-02-01 00:00:00

abstract：:A kappa-light chain from a Fab expression system was truncated by the insertion of a stop codon in the gene sequence to produce a variable light (VL) single domain antibody (dAb). Here, we describe the expression of dAb in the periplasm of Escherichia coli through fermentation in a defined media. Immunoglobulin bindin...

journal_title：Protein expression and purification

authors： Cossins AJ,Harrison S,Popplewell AG,Gore MG

更新日期：2007-02-01 00:00:00

abstract：:Protein aggregation is a main barrier hindering structural and functional studies of a number of interesting biological targets. The E6 oncoprotein of Human Papillomavirus strain 16 (E6(16)) is difficult to express under a native soluble form in bacteria. Produced as an unfused sequence, it forms inclusion bodies. Fus...

journal_title：Protein expression and purification

authors： Zanier K,Nominé Y,Charbonnier S,Ruhlmann C,Schultz P,Schweizer J,Travé G

更新日期：2007-01-01 00:00:00

abstract：:Human Fas ligand is a medically important membrane glycoprotein that induces the apoptosis of harmful cells. A new secretory expression and purification method was devised for the production of a large amount of recombinant human Fas ligand extracellular domain (hFasLECD) by Pichia pastoris. The expression plasmid con...

journal_title：Protein expression and purification

authors： Muraki M

更新日期：2006-12-01 00:00:00

abstract：:Failure to express soluble proteins in bacteria is mainly attributed to the properties of the target protein itself, as well as the choice of the vector, the purification tag and the linker between the tag and protein, and codon usage. The expression of proteins with fusion tags to facilitate subsequent purification s...

journal_title：Protein expression and purification

authors： Kurz M,Cowieson NP,Robin G,Hume DA,Martin JL,Kobe B,Listwan P

更新日期：2006-11-01 00:00:00

abstract：:Plasmids pQE-60 and pQE-30 containing 6 x His-tag sequence were used for expression of fragments of HCV structural and non-structural proteins in Escherichia coli (E. coli). The following fragments were used: core (1-98 aa), NS3 (202-482 aa), and tetramer of hypervariable region 1 (HVR1) of E2 protein. The constructed...

journal_title：Protein expression and purification

authors： Mihailova M,Fiedler M,Boos M,Petrovskis I,Sominskaya I,Roggendorf M,Viazov S,Pumpens P

更新日期：2006-11-01 00:00:00

abstract：:The 42 kDa cleavage product from the carboxyl end of the Plasmodium falciparum merozoite surface protein 1 (MSP1(42)) is an important blood-stage malaria vaccine target. Several recombinant protein expression systems have been used for production of MSP1(42) including yeast (Saccharomyces cerevisiae and Pichia pastori...

journal_title：Protein expression and purification

authors： Shimp RL Jr,Martin LB,Zhang Y,Henderson BS,Duggan P,MacDonald NJ,Lebowitz J,Saul A,Narum DL

更新日期：2006-11-01 00:00:00

abstract：:Leishmaniasis is considered by the World Health Organization to be the second most important disease caused by a protozoan parasite. Biochemical and molecular biology studies can help in the understanding of the biology of the Leishmania parasite. All protozoan parasites, including Leishmania, are unable to synthesize...

journal_title：Protein expression and purification

authors： de Oliveira AH,Ruiz JC,Cruz AK,Greene LJ,Rosa JC,Ward RJ

更新日期：2006-10-01 00:00:00

abstract：:Pyrroline-5-carboxylate reductase (P5CR) catalyzes the reduction of Delta1-pyrroline-5-carboxylate (P5C) to proline with concomitant oxidation of NAD(P)H to NAD(P)(+). The enzymatic cycle between P5C and proline is very important in many physiological and pathological processes. Human P5CR was over-expressed in Escher...

journal_title：Protein expression and purification

authors： Meng Z,Lou Z,Liu Z,Hui D,Bartlam M,Rao Z

更新日期：2006-09-01 00:00:00

abstract：:Human small nuclear (sn) RNA genes are transcribed by either RNA polymerase II or III depending upon the arrangement of their core promoter elements. Regardless of polymerase specificity, these genes share a requirement for a general transcription factor called the snRNA activating protein complex or SNAP(C). This mul...

journal_title：Protein expression and purification

authors： Hanzlowsky A,Jelencic B,Jawdekar G,Hinkley CS,Geiger JH,Henry RW

更新日期：2006-08-01 00:00:00